An increasing number of alternatives now offers new hope for the welfare of millions of animals used in biomedical research, product safety testing, and education.
While the use of alternatives doesn’t always mean eliminating animal testing altogether, the number of animals being used has been dramatically reduced in recent years, and the lives of many others have been significantly improved. The words now used almost universally by the research community and by animal protection advocates to describe alternatives are “The Three Rs” – Replacement, Reduction, and Refinement. This page clarifies these terms, describes efforts to promote “The Three Rs,” and suggests good sources of further information.
Basic Uses of Laboratory Animals
Animals in laboratories are used in many ways. Each year, an estimated 25-35 million animals worldwide serve as models for testing the safety of a wide range of consumer products, including cosmetics, drugs and vaccines, household cleaning products, pesticides, industrial chemicals, automobiles, and toys. Animals are also used in education: they are used in high school and college biology classes, as well as for teaching anatomy and surgery in veterinary and nursing schools. In addition, they are used for biomedical research on the workings of the human body and mind and for studying disease. Whether or not it is necessary to continue to use laboratory animals is a matter of opinion. However, when testing alternatives are available, the most humane stance is to forgo animal testing and use other methods.
Some believe animals are suffering needlessly since alternatives exist, and because tests on animals rarely predict human response. Others say alternatives can never replace the value of live animals in research, and public health and safety – and the future of biomedical research – would be seriously jeopardized without animal testing. The MSPCA’s position is that the truth lies somewhere between these two extremes and that support for the development of alternatives offers scientists and animal activists an excellent way to work together toward ending the need for animal use in a scientifically sound way.
What is Meant by Alternatives?
The widely-applied concept of “The Three Rs” — Replacement, Reduction, and Refinement — was originally introduced in the 1950s by William Russell and Rex Burch, British biologists who sought to lessen the suffering of laboratory animals. Though many may assume Russell and Burch’s concept specifically seeks to eliminate animal testing in favor of alternatives, the Three Rs are much broader and seek to alleviate animal suffering in a variety of ways.
“Replacement” means substituting other systems for whole animal use. Examples include human and animal cell, tissue, and organ cultures; chemical systems; blood products; computer simulations; and plastic organ models. “Reduction” means decreasing the number of animals to the minimum needed to yield accurate data and is often achieved through more strategic study planning. “Refinement” means using modern medicine to minimize or eliminate pain and distress; it also includes employing housing and husbandry techniques to enrich the captive environment to reduce boredom and promote natural behavior.
What Alternatives Are Currently in Use?
The National Cancer Institute (NCI) has replaced nearly 6 million mice per year with human cells in an in vitro (test-tube) procedure used to detect anti-cancer compounds. NCI can now test 20,000 compounds annually, saving two-thirds of the cost of the animal test.
At some veterinary schools, plastic soft-tissue organ models are replacing the dogs traditionally used to teach beginning surgery. A study conducted at the University of Illinois in 1993-94 showed that students who learned to cut and suture using these and other alternative models performed just as well in surgery on animal patients as did those trained on dogs. In 2016, a company called SynDaver began producing a line of synthetic canine models that mimic all of a dog’s body systems and can even be programmed to simulate various diseases or medical complications. Such technology allows for live animals to be replaced with realistic substitutes in veterinary teaching programs throughout the world. The U.S. Department of Defense has also developed computer models of dolphin echolocation (sonar) that will replace animals as underwater object detectors.
The classical LD50 test, used to determine toxicity, measures the dosage needed to kill 50 percent of the treated animals. Formerly used to satisfy national and international requirements for product safety testing, the LD50 required as many as 200 animals per test. One widely accepted alternative is the “limit” test, which requires only 6-10 animals. This test uses a small number of animals to determine minimum and maximum toxicity, and a limit is set on the number of doses to be tested. As a result of this and other alternatives, the number of animals used in LD50 testing has recently fallen significantly, and the LD50 test no longer represents an international testing standard.
Some scientists are using state-of-the-art medical technologies such as the MRI (magnetic resonance imaging), the MRS (magnetic resonance spectroscopy), and CAT (computer-aided tomography) scans to detect tumor growth and organ deterioration at early stages. This non-invasive imaging not only enables experiments to end more humanely, without prolonged suffering, but also reduces the number of animals needed for certain types of research.
The Draize test is used to determine the irritancy of substances such as shampoos or household cleaners that could accidentally drip or be sprayed into human eyes. It involves placing a test substance, such as a household cleaner chemical, into one eye of several rabbits, then checking the eyes at various intervals to assess any damage. An alternative to the Draize test, called the low-volume eye test (LVET) uses one-tenth of the amount of the substance. It reduces the potential for pain and discomfort,and is more predictive of possible human eye irritation, because it more closely simulates human experience than the standard Draize test.
Animal use for eye irritancy tests has fallen significantly as new in vitro tests have been developed that screen out potentially irritating substances before they are tested on animals. For example, in 2014, the EpiOcular Eye Irritation Test, which uses tissue that closely mimics the properties of human corneal tissue, was validated as an alternative eye irritation test. New and more effective pain-relieving drugs are also being used at many research institutions to alleviate post-surgical distress.
Steps are also being taken to minimize the stress of mice, the most common animals used in research. When mice undergo routine procedures (such as blood pressure measurement, injections, and blood collection), they are often held in cylindrical or cone-shaped restrainers that are inherently uncomfortable and offer no easy-to-grip surfaces for the mice. This causes them to have a continuous loss of balance, and their panic to get out of the container can result in trauma as they fight to wriggle out. In 2015, a vet tech at the University of Michigan developed the LACube, a square restrainer with a textured floor that is comfortable and safe for mice, eliminating any unnecessary stress to the animal during routine procedures.
Some research facilities have developed new ways to enrich the laboratory environment. This enables animals to express more of their normal behavior and alleviates two of the biggest problems facing laboratory animals – isolation and boredom. New caging systems now allow small monkeys to live in pairs or groups, reducing some of the stress experienced by these highly-social animals. Stones placed in frogs’ tanks and stainless steel tubes large enough to hide in are making laboratory life a little more interesting for some amphibians.
Can Alternatives Replace All Animal Tests?
The simple answer is: not yet. While the number of animals used in teaching and in product safety testing has fallen dramatically in recent years, in biomedical research – where most lab animals are used in the US today – most scientists believe it is not feasible to replace whole animals in the near term. They say we simply do not yet understand all the complexities of the human or animal body.
In fact, recent advances in genetic engineering research now threaten to dramatically increase the number of animals used in biomedical research, as scientists race to put new technology to use. New refinement techniques – the third, often- forgotten “R”– need to be developed to improve laboratory animal veterinarians’ ability to assess pain and relieve the discomfort of animals created, whether intentionally or unintentionally, with genetic abnormalities. Much work also needs to be done to increase the validation, acceptance, and use of alternatives in product safety testing by federal regulatory agencies.
Meanwhile, momentum for alternatives development continues to build. Pressure from animal-protection groups and consumers – as well as growing scientific awareness that non-whole-animal tests can produce cheaper, faster, and, often, more accurate results – will ensure the continued expansion of alternatives to laboratory animal procedures.
We believe a well-informed public is essential to improving the welfare of animals. We encourage concerned individuals to learn more about alternatives, and to communicate with elected officials, federal and state regulatory agencies, educational institutions, and industry representatives about increasing the use of alternatives in research, testing, and education.
For More Information
To learn more about alternatives, contact: The Johns Hopkins Center for Alternatives to Animal Testing, 111 Market Place, Suite 840, Baltimore, MD 21202, and the N.I.H. Interagency Coordinating Committee for the Validation of Alternative Methods (ICCVAM).